Institute for Pheromone Research
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  • Laboratory is equipped with several analytical instrument sets required for isolation, analysis and characterization of pheromones and their receptor and transporter proteins.
  • First, relatively small chemical signaling compounds must be isolated from the biological matrix such as urine. Sorptive extraction (stirbars) and dynamic head-space sampling are our common approaches. After extraction, volatile and semivolatile compounds will be desorbed into the analytical system. Typically, gas chromatography-mass spectrometry (GC-MS) is used for the analysis of volatile and semivolatile compounds. We use ion trap GC-MS and high-resolution time-of-flight GC-MS (GC-TOF) for quantification and identification. Sample introduction method is either manual or automatic thermal desorption (TDS or TDSA).
  • Highly sensitive element specific GC-Atomic Emission Detection (GC-AED) adds information, e.g., on sulfur and nitrogen containing compounds.
  • Extra identification tool is a preparational fraction collector (PFC) system connected to the GC-FID system.
  • Additionally, access to instrumentation for the analysis of non-volatile and macromolecular compounds is available. We can use a Micro-LC Ion Trap Mass Spectrometry for characterization of thermally unstable compounds.
  • Protein receptor and transporter characterization is commonly performed by gel electrophoresis followed by the MALDI-TOF analysis.
Instrument
TDSA-GC-MS




Institute for Pheromone Research
800 E. Kirkwood Ave.
Bloomington, IN 47405-7102
Phone: (812) 855-4532
Fax: (812) 855-8300
Indiana University
Last updated: April 30, 2015
Comments: phero@indiana.edu
Copyright 2002, The Trustees of Indiana University