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Methods

 

Currently, the lab employs a variety of methods, including:

  • Golgi histology and retrograde labeling techniques to visualize whole neurons for assessment of changes in morphology of dendrites and spines, which are critical determinants of neuronal function

 

 

  • Quantitative autoradiography to assess changes in receptor binding

  • In situ hybridization to assess changes in expression of mRNA for receptor subunits, which may contribute to changes in receptor function

Insitu

  • Immunohistochemistry to assess changes in amounts of receptor protein, which contribute to changes in receptor function

 

D5
GluR1
Glur1-tritc

 

  • Behavioral testing to assess stress-related changes in cognitive processes mediated by prefrontal cortex

 

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